Overexpression Of Mirna - 1441 Words

There are many evidences that demonstrates role played by miRNA in various cardio vascular diseases. In a study Dicer was deleted that led a phenotype in myocardial and vascular lineage. In cardiac myocytes miR-1 is the most populated. Expression levels of several miRNA like miR-10a, miR-145, miR-143 and miR-126100−102 In a study it showed that hypoxia had potential to induce the expression of miR-210 in endothelial cells. It was concluded that overexpression of miR-210 leads to increase in capillary like structure formation, when miR-210 was inhibited the tube formation and migration was decreased (15). In another study it was reported that miR-221 and miR-222 had antiangiogenic characteristics in endothelial cells. This study came up†¦show more content†¦Surprising, the target of both the miRNAs has the same target and functions in a similar way. Further study exposed that the EBV-infected cells escaped the apoptosis mechanism due to the expression of miR-155. This shows the potential of virus that controls the miRNA expression for its survival benefit (14). Micro RNA in neurodegenerative diseases- In the brain approximately 70% of the miRNA are expressed and most of it are neuron specific. MiRNA are important element for proper nervous system function. These miRNA plays a crucial role for brain function. Any disruption in the miRNA processing mechanism would cause diseases like ataxia in Purkinje cells, multiple sclerosis in oligodendrocyte cells, Parkinson’s disease in dopaminergic cells, Alzheimer’s disease in ÃŽ ±-calcium/calmodulin-dependent protein kinase type II (ÃŽ ±CaMKII)-expressing neurons (16). Recent studies on systemic miRNA profiling of peripheral blood mononuclear cells from Parkinson’s disease patients reported miR-30b, miR-26a, miR-26a are the miRNA that are with disease susceptibility (14). Mutation in the leucine-rich repeat serine/ threonine protein kinase2 cause negative regulation of the transitional repression by miRNA, which leads to Parkinson’s disease. Mutation in Drosha complex cause familiarShow MoreRelatedBreast Cancer : A Complex And Heterogeneous Disease Caused By Genetic Mutations782 Words   |  4 Pages(BRCA1 and BRCA2) [1]; loss or aberrant expression of the estrogen receptor (ER) [2]; human epidermal growth factor receptor (HER2) overexpression [3] ; lack of ER, progesterone receptor (PR), and epidermal growth factor receptor (EGFR) expression[4] ; high levels of receptor for the insulin-like growth factor receptor 1 (IGF-1R[5]; and expression of microRNA (miRNA) [6]. In addition to genetic alterations (gene amplifications, deletions, point mutations, and chromosome rearrangements), epigeneticRead MoreCdna Case Study1094 Words   |  5 Pagesand 787 known miRNAs were identified from cin1, CIN1-S, and WT strain, whereas 12 novel miRNAs were identified from all three strains. Significantly, differential expression of ex-sRNA exists among the three strains (Figure 9A) and the initial Gene Ontology (GO) and KEGG pathway analysis of miRNA targets is also consistent with the established role of Cin1 in intracellular trafficking (cin1 vs. WT, Figure 9B). Further annotation and screening of differentially expressed sRNA, miRNA target predictionRead MoreAnalysis Of Hepatocellular Carcinoma ( Hcc )1136 Words   |  5 Pagesmetastasis, and prognosis [43]. HCC is also characterized by aberrant expression of miRNAs. Respectively, upregulation and downregulation has been associated with invasion and metastasis, tumor progression, and drug resistance. Meng et al. proposed that aberrantly expressed miRNAs may regulate the expression of certain genes that control cell growth, migration, and invasion. In this study, the expression of miRNA in normal versus tumor tissue was investigated which led to the identification of overexpressedRead MoreEssay On Exosomes1006 Words   |  5 Pagesseries of sample, but the striking evidences provided by Melo and colleagues suggest that GPC1+ exosomes may serve as a potential diagnostic and screening biomarker to detect early stages of PC for possible curative surgery. In earlier studies, overexpression of surviving [393], and mislocalisation of plectin [394] in exosomes were also proposed as a biomarkers for PC. Moreover, it has also recently been found that a higher rate of patients with localized PC showed noticeable KRAS mutations in exosomalRead MoreCase Study Of DLBCA1185 Words   |  5 PagesABC-DLBCL, many investigators try to identify a miRNA or a set of miRNAs to use as a potential diagnostic biomarkers, for differentiation of ABC from GCB-DLBCL. Ago2-IP followed by sequencing is becoming the most advanced tool to determine the miRNA profile and to investigate functionally active miRNA, as it has been shown recently by Cullen et al. that the level of a miRNAs in total cellular lysate, it is not a clear indication of involvement of miRNAs in to the Ago2 complex [91]. In the presentRead MoreMir-423-3p And EBV Case Study789 Words   |  4 Pageshigher expression, compared to miR-423-5p, in total cellular profile in all four cell lines. Surprisingly in Ago2-IP-Seq, miR-423-5p found to be more expressed, compared to miR-423-3p, in all four cell lines. Also miR-423-5p were the most enriched miRNA in the Ago2-IP of all cell lines we note that both miR-423-5p and miR-423-3p were in Ago2-IP-seq and miR-423-3p was more expressed in total cellular profile, compared to miR-423-5p. In all four cell lines, this results were in accordance withRead MoreMirna Essay1141 Words   |  5 PagesThe original goal of this study was to identify miRNAs that are differentially expressed in the brains of HIV-infected patients with HIV-associated neurocognitive disorders (HAND). These disorders characterize a spectrum neurological syndrome that affects up to one-fourth of all HIV/AID-infected humans. Three proposed mechanism contribute to HAND, those given were gene tic host susceptibility factors, viral properties, and altered host immune responses. The first factor listed (altered host expressions)Read MoreEssay On Biogenesis1150 Words   |  5 Pagesin BC compared with CP progenitors. This signaling pathway increases the ADAR1 expression in progenitor LSCs and promotes ADAR1-mediated A-to-I editing. Furthermore, ADAR1 impairs biogenesis of tumour suppressive miRNAs, thereby contributing to cancer progression. Since the let-7 miRNA family is also related to self renewal of LSC, it led the authors to investigate the correlation between JAK2/STAT pathway and LIN28B/let-7-axis, they hypothesized that the LIN28B/let-7self-renewal axis may also beRead MoreEssay On Exosomes988 Words   |  4 Pagesseries of sample, but the striking evidences provided by Melo and colleague suggests that GPC1+ exosomes may serve as a potential diagnostic and screening biomarker to detect early stages of PC for possible curative surgery. In earlier studies, overex pression of survivin380, and mislocalisation of plectin381 in exosomes were also proposed as a biomarkers for PC. Moreover, it has also recently been found that a higher rate of patients with localized PC showed noticeable KRAS mutations in exosomal DNARead MoreSymptoms And Treatment Of Renal Disorders Essay4299 Words   |  18 PagesAbstract MicroRNAs (miRNA) are endogenously produced short non-coding regulatory RNAs that can repress gene expression by posttranscriptional mechanisms. They can therefore influence both normal and pathological conditions in diverse biological systems. Several miRNAs have been detected in kidneys where they have been found to be crucial for renal development and normal physiological functions as well as significant contributors to the pathogenesis of renal disorders such as diabetic nephropathy